Pengaruh Metode Ekstraksi terhadap Kandungan Flavonoid Total Ekstrak Etanol Daun Kelor (Moringa oleifera)
DOI:
https://doi.org/10.65117/pzqr2423Keywords:
Extraction Moringa LeavesAbstract
Moringa oleifera leaves are one of the plants that are often used by people as daily food and traditional medicine as anti-inflammatory, antioxidant, and antidiabetic. The extraction of flavonoid compounds in Moringa leaves can be maximized using the appropriate extraction method. This study aims to determine the effect of variations in maceration (M), soxhletation (S), and digestion (D) extraction methods on the total flavonoid content in moringa leaf extracts. Moringa leaves were extracted with 96% ethanol (1:5) using the M, S, D method. Qualitative tests were carried out using tube tests and quantitative tests by measuring the total flavonoid content of Moringa leaf extracts from M, S, and D using UV-Vis spectrophotometry. The quantitative data obtained were then analyzed statistically using SPSS with the One-Way ANOVA test with a 95% confidence level. Moringa leaf extracts from M, S, and D were 11,59%, 22,41%, and 10,55%, respectively. The results of the tube test showed that Moringa leaf extract contained flavonoid compounds. The total flavonoid levels obtained from the M, S, and D moringa leaf extracts were 41,544 ± 1,171 mg QE/g, 70,584 ± 1,448 mg QE/g, and 38,308 ± 0,442 mg QE/g, respectively. The Shapiro-Wilk normality test obtained M (0,512), S (0,132), and D (0,427). The Levene homogeneity test obtained a result of 0,138 and the One-Way ANOVA test obtained a result of 0,000. The soxhlet extraction method produced higher flavonoid levels than maceration and digestion. There were significant differences between the three extraction methods.
References
Afriani, N., Idiawati, N., Alimuddin, A. H., & Nawawi, J. H. H. 2016. Skrining Fitokimia Dan Uji Toksisitas Ekstrak Akar Mentawa (Artocarpus Anisophyllus) Terhadap Larva Artemia Salina. Jurnal Kimia Khatulistiwa, 5(1), 58–64.
Aminah, A., Tomayahu, N., & Abidin, Z. 2017. Penetapan Kadar Flavonoid Total Ekstrak Etanol Kulit Buah Alpukat (Persea Americana Mill.) Dengan Metode Spektrofotometri Uv-Vis. Jurnal Fitofarmaka Indonesia, 4(2), 226–230.
Berawi, K. N., Wahyudo, R., & Pratama, A. A. 2019. Potensi Terapi Moringa Oleifera (Kelor) Pada Penyakit Degeneratif. Jurnal Kedokteran Unila, 1, 210–214.
Candra, L. M. M., Andayani, Y., & Wirasisya, D. G. 2021. Pengaruh Metode Ekstraksi Terhadap Kandungan Fenolik Total Dan Flavonoid Total Pada Ekstrak Etanol Buncis (Phaseolus Vulgaris L.). Jurnal Pijar Mipa, Volume 16 No. 3, Hal. 397–405.
Fajarwati, S., Qonitah, F., & Ahwan, A. (2023). Penentuan Kandungan Flavonoid Total Dan Aktivitas Antioksidan Ekstrak Etanol Kulit Pisang Goroho (Musa Acuminafe L.). Duta Pharma Journal, 3(1), 8–21. https://doi.org/10.47701/djp.v3i1.2840
Kemenkes RI. 2017. Farmakope Herbal Indonesia Edisi II. Kementerian Kesehatan Republik Indonesia.
Marwati, Nur, S., Khairi, N., & Nursamsiar. 2022. Pengaruh Metode Ekstraksi Terhadap Aktivitas Antioksidan Ekstrak Daun Karamunting (Rhodomyrtus Tomentosa (Aiton) Hassk) Dengan Metode Dpph. Jurnal Ilmiah Farmasi Farmasyifa, 5(2), 183–191.
Nudiasari, V., Suhariyadi, & Istanto, W. 2019. Efektivitas Ekstraksi Antara Maserasi Dengan Digesti Terhadap Kadar Flavonoid Buah Naga Putih (Hylocereus Undatus). Analis Kesehatan Sains, Volume 1.
Nurulita, N. A., Sundhani, E., Amalia, I., Rahmawati, F., Nurhayati, N., & Utami, D. 2019. Uji Aktivitas Antioksidan Dan Anti Aging Body Butter Dengan Bahan Aktif Ekstrak Daun Kelor. Jurnal Ilmu Kefarmasian Indonesia, 17(1), 1–8.
Prasetyo, A. B., Imawati, M. F., & Sumadji, A. R. 2022. Pengaruh Metode Maserasi Dan Soxhletasi Terhadap Kadar Flavonoid Ekstrak Etanol Daun Kemangi (Ocimum Basilicum L). Jurnal Ilmiah Manuntung, Volume 8 No. 2, Hal. 317–321.
Putri, C. N., Rahardhian, M. R. R., & Ramonah, D. 2022. Pengaruh Metode Ekstraksi Terhadap Kadar Total Fenol Dan Total Flavonoid Esktrak Etanol Daun Insulin (Smallanthus Sonchifolius) Serta Aktivitas Antibakteri Terhadap Staphylococcus Aureus. Jpscr: Journal Of Pharmaceutical Science And Clinical Research, 7(1), 15–27.
Ramadhan, N. U., Qonitah, F., & Ariastuti, R. 2024. Uji Kandungan Fenolik Total Ekstrak Etanol Daun Dan Kulit Batang Kelor (Moringa Oleifera). Jurnal Farmasi, Kesehatan Dan Sains (Faskes), Volume 2 No. 1, Hal. 136.
Robbiyan, R., Pandapotan, M. M., & Apriani, R. 2021. Penentuan Kadar Flavonoid Dari Ekstrak Kulit Salak (Salacca Zalacca. Reinw) Berdasarkan Perbedaan Pengeringan Simplisia. Lantanida Journal, Volume 9 No. 1
Rosita, J. M., Taufiqurrahman, I., & Edyson, E. 2019. Perbedaan Total Flavonoid Antara Metode Maserasi Dengan Sokletasi Pada Ekstrak Daun Binjai (Mangifera Caesia) (Studi Pendahuluan Terhadap Proses Pembuatan Sediaan Obat Penyembuhan Luka). Dentin, 1(1).
Senduk, T. W., Montolalu, L. A. D. Y., & Dotulong, V. 2022. The Rendement Of Boiled Water Extract Of Mature Leaves Of Mangrove Sonneratia Alba. Jurnal Perikanan Dan Kelautan Tropis, 11(1), 9–15.
Syarifuddin, A. N., Purba, R. A., Situmorang, B. N., & Marbun, R. A. T. 2020. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kemangi (Ocimum Basilicum L.) Terhadap Bakteri Streptococcus Mutans. Jurnal Farmasimed (Jfm), Volume 2 No. 2, Hal. 69–76.
Susanty, Ridnugrah, N. A., Chaerrudin, A., & Yudistirani, S. A. 2019. Aktivitas Antioksidan Ekstrak Daun Kelor (Moringa Oleifera) Sebagai Zat Tambahan Pembuatan Moisturizer. Seminar Nasional Sains Dan Teknologi 2019.
Susanty, Yudistirani, S. A., & Islam, M. B. 2019. Metode Ekstraksi Untuk Perolehan Kandungan Flavonoid Tertinggi Dari Ekstrak Daun Kelor (Moringa Oleifera Lam). Konversi, 8(2), 31–36.
Tetti, M. 2014. Ekstraksi Pemisahan Senyawa Dan Identifikasi Senyawa Aktif. Jurnal Kesehatan, 7(2), 361–367.
Wang, Q., Jin, J., Dai, N., Han, N., Han, J., & Bao, B. 2016. Anti-Inflammatory Effects, Nuclear Magnetic Resonance Identification, And High-Performance Liquid Chromatography Isolation Of The Total Flavonoids. Journal Of Food And Drug Analysis, 24(2), 385–391.
Wicaksono, S., Santoso, J., & Prabandari, S. 2023. Pengaruh Perbedaan Metode Ekstraksi Terhadap Kadar Flavonoid Total Ekstrak Daun Kelor (Moringa Oleifera L.) Dengan Metode Spektrofotometri Uv-Vis. Jurnal Ilmiah Farmasi.
Downloads
Published
Issue
Section
License
Copyright (c) 2026 fadilah qonitah, Ahwan (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Jurnal Farmasi Sains dan Teknologi © 2023 by Jurnal Farmasi Sains dan Teknologi (JFST) is licensed under CC BY-SA 4.0 
